Resumo Allium sativum L. é uma erva da família Alliaceae com sabor e aroma específicos e propriedades medicinais e nutracêuticas amplamente comercializada em diversos países. O Brasil é um dos maiores importadores de alho do mundo, apesar da sua produção ser restrita e limitada ao consumo interno. Assim, explorar a diversidade genética do alho comercial conservado em bancos de germoplasma é essencial para fornecer informações genéticas adicionais acerca dessa cultura economicamente importante. Uma ferramenta adequada para esse fim é a caracterização citogenética desses acessos. Este estudo teve como objetivo caracterizar a diversidade citogenética entre sete acessos de alho de um Banco de Germoplasma no Brasil. Os cariótipos foram obtidos por coloração convencional e com os fluorocromos de cromomicina A3 (CMA) e 4,6-diamidino-2-fenilindol (DAPI). Todos os acessos analisados apresentaram número cromossômico 2n = 16, fórmula cariotípica 6M + 2SM, cariótipos simétricos, núcleos reticulados em intérfase e cromossomos com condensação uniforme da cromatina da prófase para a metáfase. A coloração com fluorocromos mostrou diferenças na quantidade e distribuição de heterocromatina ao longo dos cromossomos e entre os acessos estudados. Com base no padrão de distribuição desses pequenos polimorfismos, foi possível separar os sete acessos em três grupos. Também foi possível diferenciar individualmente alguns dos acessos. Um dos resultados obtidos mostrou distensão heteromórfica da região organizadora nucleolar observada nos pares dos cromossomos 6 ou 7 com características peculiares. Foi sugerido, por exemplo, que o bloco heteromórfico de heterocromatina (CMA +++ / DAPI-) no cromossomo 6 do acesso “Branco Mineiro Piauí” pode ser usado como um marcador para identificar esse genótipo ou pode estar associado a algum caráter de interesse econômico.

Abstract Allium sativum L. is an herb of the Alliaceae family with a specific taste and aroma and medicinal and nutraceutical properties that are widely marketed in several countries. Brazil is one of the largest importers of garlic in the world, despite of its production is restricted and limited to internal consumption. Thus, explore the genetic diversity of commercial garlic conserved at germplasm banks is essential to generate additional genetic information about its economically important crop. A suitable tool for this purpose is the cytogenetic characterisation of these accessions. This study aimed to characterise the cytogenetic diversity among seven accessions of garlic from a Germplasm Bank in Brazil. The karyotypes were obtained by conventional staining and with chromomycin A3 (CMA) and 4,6-diamidino-2-phenylindole (DAPI) fluorochromes. All accessions analysed showed chromosome number 2n = 16, karyotype formula 6M+2SM, symmetrical karyotypes, reticulate interphase nuclei, and chromosomes with uniform chromatin condensation from prophase to metaphase. The fluorochromes staining showed differences in the amount and distribution of heterochromatin along the chromosomes and between accessions studied. Based on the distribution pattern of these small polymorphisms, it was possible to separate the seven accessions into three groups. It was also possible to differentiate some of the accessions individually. One of the results obtained showed a heteromorphic distension of the nucleolar organiser region observed on the chromosome pairs 6 or 7 with peculiar characteristics. It was suggested for example, that the heteromorphic block of heterochromatin (CMA+++/DAPI-) on chromosome 6 of the “Branco Mineiro Piauí” accession can be used as a marker to identify this genotype or may be associated with some character of economic interest.

Shape memory alloys (SMA) are now widely studied in academia and have increasing usage in industry, as they allow efficient performance compared to mechanic actuators with reduced dimensions and the possibility of sensing temperature through the material itself. A subclass of the SMAs are the alloys with magnetic shape memory, still little explored as intelligent materials in Brazil. They combine the best properties of SMAs and common magnetostrictive materials. The magnetic shape memory alloys (MSMA) can be activated not only by the presence of a thermal stimulus, but also by the presence of an applied magnetic field. In this research, the polycrystalline magnetic shape memory alloys Ni51.3Mn24Ga24.7 and Ni54Mn21Ga25 were manufactured and characterized. Differential scanning calorimetry, X-Ray diffraction, energy dispersive X-ray spectrometry and scanning electron microscopy analyzes were performed to thermoanalytically and crystallographically characterize the alloys. Magnetic characterizations were also performed using Magnetic force microscopy. The results of the characterizations showed that the production process and the heat treatment were satisfactory for the fabrication of the ferromagnetic polycrystalline alloys. Besides that, the compositions Ni51.3Mn24Ga24.7 and Ni54Mn21Ga25, presented austenitic and martensitic 5M structures, respectively, at room temperature and it was possible to observe the alignment of the magnetic domains after magnetization.

Resumo A liberação de água do hipolímnio do reservatório, baixa concentração de oxigênio e a regulação antropogênica do fluxo do rio podem afetar a reprodução de peixes, principalmente espécies migradoras. No entanto, pouco se sabe sobre os efeitos dessas mudanças nas condições da água em espécies não migradoras. A reprodução de Acestrohynchus lacustris foi avaliada em duas seções do rio São Francisco. Seção 1, localizada imediatamente a jusante da barragem de Três Marias e seção 2, localizada na confluência dos rios São Francisco e Abaeté. Para isso, foram obtidos os parâmetros físico-químicos da água de cada seção do estudo. Além disso, dados biométricos e índices biológicos de todos os peixes capturados foram obtidos. Adicionalmente, nas fêmeas foram medidos os diâmetros dos folículos vitelogênicos e a fecundidade. Temperatura, oxigênio dissolvido e fluxo apresentaram valores mais baixos na seção 1. Os peixes capturados nesta seção apresentaram menores valores de IGS em ambos os sexos, e as fêmeas apresentaram menores valores de fecundidade e diâmetro dos folículos. Essa espécie apresentou atividade reprodutiva nas duas seções analisadas, porém, na seção 1, onde os parâmetros da água apresentam piores condições para o processo reprodutivo de peixes, a capacidade reprodutiva de A. lacustris foi afetada negativamente.

Abstract The release of water from the reservoir hypolimnion, lower concentration of oxygen and the anthropogenic regulation of the river flow, could affect the reproduction of fish, especially migratory species. However, little is known about the effects of these changes in water on non-migratory species. In this sense, the reproduction of Acestrohynchus lacustris was evaluated in two sections of São Francisco River, Minas Gerais, Brazil. Section 1, located immediately downstream from Três Marias Dam (18°09’31.65”S and 45°13’36.00”W) and section 2, located at the confluence of the São Francisco and the Abaeté Rivers (18°02’47.78”S and 45°10’57.95”W). For this, we obtained the physico-chemical parameters of water of each study section. Additionally, biometric data and biological indices of all specimens were measured. Fecundity and follicles diameters were measured in females. Temperature, dissolved oxygen and flow showed lower values in section 1. Fish captured in this section, had lower values of GSI in both sexes, and females presented decreased values of fecundity and follicles diameter. This species showed reproductive activity in the two sections analyzed, however, in section 1 where the temperature and dissolved oxygen presented significant lower values, the reproductive capacity of A. lacustris, was negatively affected.

Resumo O presente trabalho apresenta o estudo experimental de pilares de concreto armado reforçados com parafusos chumbadores e acréscimo de camada com 35 mm de concreto autoadensável na face comprimida. Nove pilares, com alturas de 2000 mm, foram submetidos à flexão composta reta com excentricidade inicial de 60 mm, assim discriminados: um pilar original (PO) com seção transversal de 120 mm x 250 mm; um pilar de referência (PREF) com seção transversal de 155 mm x 250 mm e sete pilares, inicialmente moldados com seção transversal de 120 mm x 250 mm, e após, reforçados através da adição de camada de reforço de 35 mm e diversas configurações de posições de parafusos chumbadores na face comprimida. Exceto o pilar original (PO), os pilares foram submetidos à excentricidade inicial de carga de 42,5 mm, devido ao acréscimo da camada de reforço na face comprimida. Apesar de não ocorrer ruptura dos parafusos chumbadores, não foi possível evitar o desplacamento da camada de reforço. Os resultados obtidos indicam que é possível a reabilitação estrutural de pilares de concreto armado com o uso da metodologia de reforço empregada, obtendo ganho de capacidade de carga média de 271 % em relação ao pilar original (PO).

Abstract This paper presents the experimental study of eccentrically loaded reinforced concrete columns with an added 35 mm self-compacting concrete jacket attached to the column’s most compressed face using wedge bolts. Nine columns with a 2000 mm height were tested under compression and one-way bending until failure. Columns were denominated as original column (PO) with a cross section of 120 mm x 250 mm; reference column (PR) with a cross section of 155 mm x 250 mm, and seven columns with an initial cross section of 120 mm x 250 mm and later reinforced by the addition of 35 mm self-compacting concrete layer and various configurations of wedge bolts. Except for the original column PO, the columns were submitted to a 42.5 mm load eccentricity due to the added concrete layer at the compressed face. Although failure of the wedge bolts did not occur, it was not possible to prevent detachment of the added layer. The results indicate that it is possible to structurally rehabilitate reinforce concrete columns with the use of the strengthening methodology used in this research, resulting in average ultimate load capacity gains of 271% compared to original column’s ultimate load.

The objective of this study was to investigate the effect of whole body vibration (WBV) exercise on oxidative stress markers in a group of women with fibromyalgia (FM) compared to a group of healthy women (CT). Twenty-one women diagnosed with FM and 21 age- and weight-matched healthy women were enrolled the study. Plasma oxidative stress markers (primary outcomes) were evaluated at rest and after WBV, and included thiobarbituric acid reactive substances (TBARS), iron reduction capacity (FRAP), superoxide dismutase antioxidant enzymes activity (SOD), and catalase (CAT). At rest, the FM group had higher TBARS (P<0.001) and FRAP (P<0.001), and lower CAT (P=0.005) compared to the CT. In the CT group, the WBV had no effect on TBARS (P=0.559) and FRAP (P=0.926), whereas it increased both SOD (P<0.001) and CAT (P<0.001). In the FM group, the WBV reduced TBARS (p <0.001), FRAP (P<0.001), and CAT (P=0.005), while it increased SOD (P=0.019). There was an interaction effect (moments vs groups) in the TBARS (effect size=1.34), FRAP (effect size=0.93), CAT (effect size=1.45), and SOD (effect size=1.44) (P<0.001). A single trial of WBV exercise improved all oxidant and antioxidant parameters towards a greater adaptation to the stress response in FM women.

Resumo A inclusão de fibras de aço em sistemas de ligações laje-pilar se torna interessante uma vez que as fibras podem melhorar a ductilidade e capacidade de absorção de energia do concreto. O uso das fibras melhora o desempenho dessas lajes, seja pelo aumento da resistência, seja pela modificação do modo de ruptura. O objetivo deste trabalho é apresentar uma análise experimental para avaliação do efeito da punção em lajes lisas de concreto armado com adição de fibras de aço e armaduras de cisalhamento. Para isso, oito lajes quadradas, 1800 mm por 1800 mm por 130 mm, foram carregadas axialmente até a ruptura. Os modelos foram divididos em dois grupos, dependendo do tipo de concreto utilizado (com ou sem fibras de aço). O volume de fibra de aço utilizado nas lajes do segundo grupo foi de 0,9%. Cada grupo foi composto por quatro modelos: um sem armadura de cisalhamento e três com armadura de cisalhamento (studs) distribuídos radialmente em torno do pilar. O uso de fibras de aço aumentou a capacidade resistente das lajes lisas em todos os modelos testados. Em um dos modelos, a associação de fibras de aço com armadura de cisalhamento alterou o modo de ruptura da laje de externo às armaduras de cisalhamento para ruptura interna à região armada.

Abstract Steel fibers in reinforced concrete increase the performance of slab-column connection once they increase ductility and energy absorption capacity of the concrete. The use of fibers in flat slabs may increase strength and change the mode of failure. The objective of this work is to present an experimental evaluation of punching shear strength of reinforced concrete flat slab with steel fibers and punching shear reinforcement. Eight square slabs, size 1800 mm by 1800 mm by 130mm, were loaded until failure by punching shear around the column. The models were divided in two groups, depending on the type of the concrete used (with or without steel fibers). The steel fiber volume used in the slabs of second group was of 0.9%. Each group was composed of four slabs: one without shear reinforcement and three with shear reinforcement (studs) distributed radially around the column. The use of steel fibers increased the ultimate strength of all flat slabs. In one of the slabs, the association of steel fibers with shear reinforcement changed the failure surface from outside to inside the punching shear reinforcement region.

Resumo Este trabalho apresenta os resultados dos ensaios de 5 lajes de concreto armado (415 cm x 415 cm x 7 cm) com o objetivo de se verificar experimentalmente a possibilidade de reforço pela face superior de lajes de concreto armado e também de se avaliar a aderência entre o concreto antigo da laje e a camada do concreto do reforço. As principais variáveis foram as deficiências da resistência do concreto e de armadura. A laje L1, de referência, foi ensaiada até a ruptura e as demais até um limite de carregamento quando foram reforçadas e reensaiadas até a ruptura. Todas as lajes romperam por flexão e nas lajes armadas com uma taxa mínima de armadura, o reforço elevou em até 30% a carga de ruptura em relação a uma laje similar sem reforço, independentemente da resistência original do concreto. Em todos os ensaios das lajes reforçadas não se detectou descolamentos ou evidências de perda de aderência entre o concreto antigo e o concreto do reforço.

Abstract The current study presents the results of tests conducted in 5 reinforced concrete slabs (415 cm x 415 cm x 7 cm) in order to experimentally check the possibility of reinforcing their upper surface, as well as to assess the adhesion between the old and the reinforcing concrete layers in the slab. The main variables were the concrete and reinforcement strength deficiencies. Reference slab “L1” was tested until reaching the failure load, whereas the others were tested until reaching certain load limit, reinforced and retested until reaching the failure load. All slabs failed under bending. The strengthening increased the failure load by 30% in slabs reinforced at minimum reinforcement rate when they were compared to similar non-reinforced slabs, regardless of the original concrete strength. None of the tests conducted in the reinforced slabs showed detachments or evidence of adhesion loss between the old and reinforcing concretes.

Resumo Este trabalho apresenta estudo sobre o comportamento de vigas de concreto armado reforçadas à flexão, pela adição de concreto e barras de aço na região tracionada e chumbadores de expansão na ligação viga-reforço, submetidas a carregamento cíclico. O programa experimental incluiu ensaios em seis vigas de concreto armado em escala real, simplesmente apoiadas, inicialmente com seção transversal retangular com 150 mm de largura e 400 mm de altura, comprimento entre os apoios de 4000 mm e comprimento total de 4500 mm. Todas as vigas, depois de receber dois ciclos de carga estática, de modo a criar uma condição de pré-fissuração, foram reforçadas à flexão por encamisamento parcial e, em seguida, submetidas a uma carga cíclica até ao final de 2x106 ciclos ou da ocorrência de ruptura por fadiga. Após a aplicação das cargas cíclicas, as vigas que não romperam por fadiga foram submetidas a uma carga estática até a ruptura. As principais variáveis foram a condição de interface de ligação entre viga e reforço (lisa ou rugosa), a taxa de armadura de flexão na viga e no reforço, e amplitude do carregamento cíclico. Com base nos resultados obtidos nos ensaios e em estudos anteriores de vigas semelhantes testadas apenas com carga estática, é feita uma discussão do comportamento dessas vigas reforçadas e apresentada uma proposta para dimensionamento da ligação viga-reforço, para os casos de carregamento predominantemente estático e cíclico.

Abstract This work presents a study on the behavior of reinforced concrete beams strengthened in bending by the addition of concrete and steel on their tension side and having expansion bolts as shear connectors at the junction between the beam and the jacket, subjected to a cyclic loading. The experimental program included tests on six full scale reinforced concrete beams, simply supported, initially with rectangular cross section 150 mm wide and 400 mm high, span of 4000 mm and total length of 4500 mm. All the beams, after receiving two cycles of static loading in order to create a pre-cracking condition, were strengthened in bending by partial jacketing and then subjected to cyclic loading until the completion of 2x106 cycles or the occurrence of fatigue failure. Following the cyclic loading, the beams that did not fail by fatigue were subjected to a static load up to failure. The main variables were the beam-jacket interface condition (smooth or rough), the flexural reinforcement ratio in the beam and in the jacket, and cyclic load amplitude. On the basis of the obtained test results and the results of previous studies of similar beams tested only under static loading, the behavior of the strengthened beams is discussed and a proposal for the beam-jacket connection design is presented, for the cases of predominantly static and cyclic loading.

Hypertension is characterized by a pro-inflammatory status, including redox imbalance and increased levels of pro-inflammatory cytokines, which may be exacerbated after heat exposure. However, the effects of heat exposure, specifically in individuals with inflammatory chronic diseases such as hypertension, are complex and not well understood. This study compared the effects of heat exposure on plasma cytokine levels and redox status parameters in 8 hypertensive (H) and 8 normotensive (N) subjects (age: 46.5±1.3 and 45.6±1.4 years old, body mass index: 25.8±0.8 and 25.6±0.6 kg/m2, mean arterial pressure: 98.0±2.8 and 86.0±2.3 mmHg, respectively). They remained at rest in a sitting position for 10 min in a thermoneutral environment (22°C) followed by 30 min in a heated environmental chamber (38°C and 60% relative humidity). Blood samples were collected before and after heat exposure. Plasma cytokine levels were measured using sandwich ELISA kits. Plasma redox status was determined by thiobarbituric acid reactive substances (TBARS) levels and ferric reducing ability of plasma (FRAP). Hypertensive subjects showed higher plasma levels of IL-10 at baseline (P<0.05), although levels of this cytokine were similar between groups after heat exposure. Moreover, after heat exposure, hypertensive individuals showed higher plasma levels of soluble TNF receptor (sTNFR1) and lower TBARS (P<0.01) and FRAP (P<0.05) levels. Controlled hypertensive subjects, who use angiotensin-converting-enzyme inhibitor (ACE inhibitors), present an anti-inflammatory status and balanced redox status. Nevertheless, exposure to a heat stress condition seems to cause an imbalance in the redox status and an unregulated inflammatory response.

Este artigo apresenta um estudo do comportamento de pilares de concreto armado reforçados por encamisamento parcial, com o uso de uma camada de 35 mm de concreto auto adensável na face mais comprimida submetidos à flexo-compressão até à ruptura. Foram utilizados parafusos conectores como armadura de ligação entre as duas camadas de concreto de diferentes idades. Foram ensaiados 7 pilares de 2000 mm de altura, sendo 2 peças concretadas monoliticamente nomeadas de PO (pilar original) e PR (pilar de referência). Os outros 5 pilares foram reforçados utilizando uma camada de 35 mm de concreto moldada na face submetida a maior compressão. O pilar PO tinha uma seção transversal retangular de 120x 250 mm e as demais colunas 155 x 250 mm após executado o reforço. Os resultados alcançados mostram que os pilares reforçados atingiram uma resistência de aproximadamente três vezes maior com relação ao pilar PO, demostrando eficiência da técnica utilizada. Os pilares apresentaram o desplacamento da camada de reforço nas cargas finais, com tendências ao esmagamento do concreto e escoamento do aço.

This article presents the study of reinforced concrete columns strengthened using a partial jacket consisting of a 35mm self-compacting concrete layer added to its most compressed face and tested in combined compression and uniaxial bending until rupture. Wedge bolt connectors were used to increase bond at the interface between the two concrete layers of different ages. Seven 2000 mm long columns were tested. Two columns were cast monolithically and named PO (original column) e PR (reference column). The other five columns were strengthened using a new 35 mm thick self-compacting concrete layer attached to the column face subjected to highest compressive stresses. Column PO had a 120mm by 250 mm rectangular cross section and other columns had a 155 mm by 250mm cross section after the strengthening procedure. Results show that the ultimate resistance of the strengthened columns was more than three times the ultimate resistance of the original column PO, indicating the effectiveness of the strengthening procedure. Detachment of the new concrete layer with concrete crushing and steel yielding occurred in the strengthened columns.

Although it is well known that physical training ameliorates brain oxidative function after injuries by enhancing the levels of neurotrophic factors and oxidative status, there is little evidence addressing the influence of exercise training itself on brain oxidative damage and data is conflicting. This study investigated the effect of well-established swimming training protocol on lipid peroxidation and components of antioxidant system in the rat brain. Male Wistar rats were randomized into trained (5 days/week, 8 weeks, 30 min; n=8) and non-trained (n=7) groups. Forty-eight hours after the last session of exercise, animals were euthanized and the brain was collected for oxidative stress analysis. Swimming training decreased thiobarbituric acid reactive substances (TBARS) levels (P<0.05) and increased the activity of the antioxidant enzyme superoxide dismutase (SOD) (P<0.05) with no effect on brain non-enzymatic total antioxidant capacity, estimated by FRAP (ferric-reducing antioxidant power) assay (P>0.05). Moreover, the swimming training promoted metabolic adaptations, such as increased maximal workload capacity (P<0.05) and maintenance of body weight. In this context, the reduced TBARS content and increased SOD antioxidant activity induced by 8 weeks of swimming training are key factors in promoting brain resistance. In conclusion, swimming training attenuated oxidative damage and increased enzymatic antioxidant but not non-enzymatic status in the rat brain.

Chronic exposure to cigarette smoke seems to be related to an increase of pro-inflammatory cytokines, oxidative stress and changes in muscular and physical performances of healthy smokers. However, these parameters have not yet been evaluated simultaneously in previous studies. The participants of this study were healthy males divided into two groups: smokers (n=20) and non-smokers (n=20). Inflammation was evaluated by measuring plasma levels of the cytokines IL-10, IL-6 e TNF-α, and of the soluble receptors sTNFR1 and sTNFR2. Oxidative stress was evaluated by determination of thiobarbituric acid reactive substances (TBARS) plasma levels, total antioxidant capacity of plasma and erythrocytes activity of the antioxidant enzymes superoxide dismutase (SOD) and catalase. Muscular performance was evaluated by measuring the peak torque of knee flexors and extensors, and by determining the total work of the knee extensors. Physical performance was assessed by measuring the peak oxygen uptake (VO2 peak), the maximum heart rate (HRmax) and the walking distance in the shuttle walking test. Smokers showed an increase in the levels of the sTNFR1 and TBARS and a decrease in the total antioxidant capacity of plasma, in the catalase activity and in the total work (P<0.05). IL-6, IL-10, sTNFR2, SOD, peak torque, VO2 peak, HRmax and walking distance were similar between groups. Smokers presented increased oxidative stress and skeletal muscle dysfunction, demonstrating that the changes in molecular and muscular parameters occur simultaneously in healthy smokers.

RESUMODiversos fatores predisponentes são descritos para as afecções mamárias ou distúrbios secundários que comprometem a qualidade e produtividade de leite de fêmeas nas diferentes espécies. As características fenotípicas do úbere são consideradas na avaliação econômica de uma fêmea caprina, tanto pelo potencial de produção como pelo registro genealógico dessa fêmea. A limitação de estudos correlacionando essas características com a saúde do úbere gera a dúvida a respeito do significado da conformação do mesmo à saúde e produtividade da glândula mamária. Sendo assim, este estudo teve como objetivo relacionar os parâmetros da conformação do úbere com a celularidade da glândula mamária aferida pelo teste California Mastitis Test (CMT) e contagem de células somáticas (CCS) em 80 cabras da raça Saanen sem alterações no exame clínico da glândula mamária nem no teste de Tamis. Observou-se que a maioria dos parâmetros fenotípicos de úbere não influenciou a CCS, sendo considerados puramente estéticos. A circunferência e profundidade de úbere demonstraram correlação negativa com a celularidade e, por serem características de herdabilidade moderada a alta, podem ser parâmetros considerados para seleção genética de caprinos.

ABSTRACTSeveral predisposing factors are described for mammary diseases or secondary disorders that compromise the quality and productivity of milk from females in different species. The phenotypic characteristics of the udder are considered in the economic evaluation of a female goat, whether for production potential, or as the genealogical record for these females. The limitation of studies correlating these features with the udder health raises doubt about the meaning of the conformation to the health and productivity of the mammary gland. Therefore, this study aimed to list the parameters of the udder conformation with the cellularity of the mammary gland checked by California Mastitis Test (CMT) and somatic cell count (SCC) in 80 Saanen goats without alterations in the clinical examination of the mammary gland or the Tamis test. It was observed that most of the phenotypic parameters of the udder did not influence the CCS, being considered purely esthetic. The circumference and udder depth showed negative correlation with the cellularity and moderate to high heritability traits can be considered parameters for genetic selection of goats.

Para que um elemento estrutural reforçado por encamisamento tenha um desempenho satisfatório é necessário garantir a transferência de esforços na região da junta do concreto antigo (substrato) com o concreto do reforço. O desplacamento entre o concreto adicionado (material do reforço) e o concreto antigo deve ser evitado quando a peça reabilitada é colocada novamente em serviço. Foram ensaiados oito pilares de concreto armado, submetidos à flexo-compressão reta, reforçados com concreto auto adensável e chumbadores metálicos cruzando a interface de ligação entre os concretos moldados em idades distintas, para evitar o desplacamento entre estes materiais. Dos oito pilares ensaiados, dois eram referências e foram denominados de PO (pilar original) e PR. (pilar referência). Os outros seis foram reforçados acrescentando-se uma camada de concreto auto adensável de 35 mm à face comprimida. O pilar PO, tinha seção transversal de 120 x 250 mm, e forneceu informações sobre o comportamento da peça antes da execução do reforço. O pilar PR tinha seção transversal de 155 x 250 mm, e forneceu informações sobre o comportamento de uma peça monolítica de seção idêntica a dos pilares após o reforço. Para executar o reforço, inicialmente escarificou-se, por hidrojateamento, a zona de interface a ser formada pelo contato entre o concreto do reforço e do substrato. Posteriormente, fizeram-se furos ao longo desta superfície, onde foram inseridos os chumbadores mecânicos responsáveis por aumentar a aderência entre os concretos. A diferença entre os seis pilares reforçados consistia justamente na variação da posição e da quantidade dos chumbadores usados em cada peça. Os resultados obtidos indicaram que a quantidade e a posição destes chumbadores interferiram significativamente na capacidade portante dos pilares reforçados, pois retardavam a ocorrência de uma ruptura prematura por desplacamento entre os concretos do reforço e do substrato.

Strengthening of reinforced concrete columns by jacketing is one of the most common structural rehabilitation techniques in Brazil. For adequate performance, it is necessary, among others, to avoid detachment of the new concrete layer (strengthening material) from the old concrete substrate when the strengthened member is again in service conditions. This paper describes the test results of eight reinforced concrete rectangular columns subjected to combined compression and one-axis bending to evaluate the efficiency of using sleeve wedge bolts across the new concrete/old concrete interface to avoid detachment. The strengthening technique, in this case, consists of adding a layer of self-compacting concrete to one face of the column. Two columns tested were monolithic and named PO (original column) e PR (reference column). The other six columns were strengthened using a new 35 mm thick self-compacting concrete layer attached to the column face subjected to highest compressive stresses. Column PO had a 120mm by 250 mm rectangular cross section and its results gave information about column behavior without the use of strengthening. Column PR had a 155mm by 250 mm rectangular cross section and its cross section dimensions matched the strengthened columns but it was cast monolithically. To improve bond conditions between the existing concrete and the new concrete, the concrete surface was roughened and the outermost aggregate was exposed using hydro jetting. Holes along the concrete surface were made to insert the wedge bolts responsible for increasing the bond between the two concrete surfaces. The difference among the six strengthened columns was the position and amount of bolts used. Results indicate that the position and amount of the bolts alters significantly the strength capacity of the columns, since premature rupture by concrete detachment was delayed.

São investigados o comportamento estrutural e a resistência última à punção de ligações laje-pilar de regiões internas das lajes lisas, com um furo adjacente ao pilar, e com ou sem transferência de momento fletor da laje ao pilar. As principais variáveis foram: a existência ou não de furo, a taxa e a distribuição da armadura de flexão, a direção e o sentido do momento transferido e a excentricidade de carga (relação M(momento transferido ao pilar)/V(força cortante)) na ligação, igual a 0,50 m ou 0,25 m. Foram ensaiadas 7 (sete) ligações internas laje-pilar e analisados os resultados das cargas últimas, fissuração, deslocamento vertical, deformações das armaduras de flexão e do concreto. A existência de furo adjacente ao menor lado do pilar, a taxa e a distribuição da armadura de flexão, a variação da relação Mu/Vu em função do carregamento, e, por conseguinte, da excentricidade, influenciaram o comportamento e a carga de ruptura das lajes. Os resultados experimentais foram comparados com os estimados pelas normas: CEB-FIP/MC1990 [7], EC2/2004 [12], ACI-318:2011 [1] e NBR 6118:2007 [5]. O ACI [1] e o EC2 [12] apresentaram estimativas mais conservadoras, embora tenham apresentado algumas estimativas contra a segurança. A NBR 6118:2007 [5], apesar de baseada em parte no EC2 [12], apresentou estimativas menos conservadoras e com um número maior de estimativas contra a segurança.

The structural behavior and the ultimate punching shear resistance of internal reinforced concrete flat slab-column connections, with one hole adjacent to the column, with or without flexural moment transfer of the slab to the column was investigated. Main variables were: the existence whether or not hole, flexural reinforcement layout and ratio, the direction and sense of the moment transferred and the eccentricity of the load (M (moment transferred to column) / V (shear)) ratio at the connection - 0,50 m or 0,25 m. Seven internal slab-column joining were tested and ultimate loads, cracking, deflections, concrete and reinforcement strains were analyzed. The existence of hole adjacent to the smaller column dimension, the hole dimension, flexural reinforcement rate and placing, the variation of relation Mu/Vu in function of the load, and, than, of eccentricity of the load, influenced the slabs behavior and rupture load. Test results were compared with the estimations from CEB-FIP/MC1990 [7], EC2/2004 [12], ACI-318:2011 [1] and NBR 6118:2007 [5]. ACI [1] and EC2 [12] presented most conservative estimates, although have presented some non conservative estimates. Brazilian NBR [5], even though being partly based in EC2 [12], presented smaller conservative estimates and more non conservative estimates. A modification on all codes is proposed for taking in account the moment caused by the eccentricity at the critical perimeter for slabs with holes.